abstract：:The mechanisms by which adenosine regulates the inflammatory reaction are poorly characterized. In this study, we investigated the effects of adenosine on neutrophil actin polymerization elicited by the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) or IgG-opsonized yeast particles. We used bodipy-...

journal_title：European journal of cell biology

authors： Zalavary S,Bengtsson T

更新日期：1998-02-01 00:00:00

abstract：:This paper explores the mode of action of the tranquillizers chloral hydrate and diazepam during fertilization and mitosis of the first reproductive cell cycles in sea urchin eggs. Most striking effects of these drugs are the alteration of centrosomal material and the abnormal microtubule configurations during exposur...

journal_title：European journal of cell biology

authors： Schatten H,Chakrabarti A

更新日期：1998-01-01 00:00:00

abstract：:When epithelial cells reach confluency in vitro, a number of energy-requiring activities such as growth and motility are contact-inhibited. We investigated the possible role of the E-cadherin/catenin complex, which acts as an invasion suppressor, in contact inhibition. Three strategies for modulation of the complex we...

journal_title：European journal of cell biology

authors： Bracke ME,Depypere H,Labit C,Van Marck V,Vennekens K,Vermeulen SJ,Maelfait I,Philippé J,Serreyn R,Mareel MM

更新日期：1997-12-01 00:00:00

abstract：:In this study we have used primary cultures of porcine superior cervical ganglia as a model system to study exo-endocytosis in sympathetic neurons. Pure neuronal cultures with a defined noradrenergic phenotype can be obtained when antimitotics are included in the culture medium, and the high yield from prenatal piglet...

journal_title：European journal of cell biology

authors： Annaert WG,Partoens P,Slembrouck D,Bakker A,Jacob W,De Potter WP

更新日期：1997-11-01 00:00:00

abstract：:Previous studies have shown that caffeine treatment at 20 degrees C causes the intermediate compartment protein p58 to redistribute from the Golgi region without affecting the localization of the Golgi stack protein mannosidase II (J. Jäntti, E. Kuismanen, J. Cell Biol. 120, 1321-1335 (1993). Here we have dissected fu...

journal_title：European journal of cell biology

authors： Jäntti J,Saraste J,Kuismanen E

更新日期：1997-10-01 00:00:00

abstract：:Phagosomes with pathogenic mycobacteria, or with hydrophobic polystyrene beads of 1 micron in size, do not mature, but remain fusogenic towards early endosomes and do not fuse with lysosomes (de Chastellier et al. Eur. J. Cell Biol. 68, 167-182 (1995)). Both types of phagocytic particles display a close apposition to ...

journal_title：European journal of cell biology

authors： de Chastellier C,Thilo L

更新日期：1997-09-01 00:00:00

abstract：:Endothelial cell-specific molecules are potential targets for new therapeutic strategies in the control of inflammatory reactions, immune responses and neoangiogenesis. We describe the production and characterization of MEC 14.7, a monoclonal antibody directed to murine endothelial cells recognizing a glycosylated pro...

journal_title：European journal of cell biology

authors： Garlanda C,Berthier R,Garin J,Stoppacciaro A,Ruco L,Vittet D,Gulino D,Matteucci C,Mantovani A,Vecchi A,Dejana E

更新日期：1997-08-01 00:00:00

abstract：:The realization that a highly conserved family of membrane proteins are localized to transport vesicles and selectively interact with proteins anchored at appropriate target sites of membrane fusion inspired a simple and compelling explanation of how proteins might be transferred and segregated within the cell, the "S...

journal_title：European journal of cell biology

authors： Terrian DM,White MK

更新日期：1997-07-01 00:00:00

abstract：:Cytolocalization of stomatin, an integral membrane protein also called erythrocyte band 7.2b, was investigated in a human epithelial cell line in which the expression of this protein is up-regulated after treatment with interleukin-6 and dexamethasone. A monoclonal antibody against stomatin was used to perform immunof...

journal_title：European journal of cell biology

authors： Snyers L,Thinès-Sempoux D,Prohaska R

更新日期：1997-07-01 00:00:00

abstract：:We have examined the importance of cell surface heparan sulfate proteoglycans (HSPG) in fibroblast growth factor (FGF) signaling. 3T3 cells grown under conventional conditions were much more sensitive to FGF-2 compared to FGF-1. However, cells were equally sensitive to FGF-1 and FGF-2 using conditions which reduced th...

journal_title：European journal of cell biology

authors： Zhou FY,Owens RT,Hermonen J,Jalkanen M,Höök M

更新日期：1997-06-01 00:00:00

abstract：:The uptake mechanism of pertussis toxin (PT) in CHO and insulin-producing HIT-T15 cells was studied. By electron microscopy after direct labeling of the toxin with gold particles, PT was found to be taken up by receptor-mediated endocytosis. The presence of active pertussis toxin in the Golgi complex was shown by subc...

journal_title：European journal of cell biology

authors： el Bayâ A,Linnemann R,von Olleschik-Elbheim L,Robenek H,Schmidt MA

更新日期：1997-05-01 00:00:00

abstract：:The organelles of the endocytic and autophagic pathways were studied in HeLa cells using immunoelectron microscopy and stereological techniques. In the absence of autophagic stimulation, characteristic particulate structures containing closely packed layers of membrane received the fluid-phase marker horseradish perox...

journal_title：European journal of cell biology

authors： Lucocq J,Walker D

更新日期：1997-04-01 00:00:00

abstract：:Epithelial cell lines can be grown on filter supports and form polarized monolayers with distinct basolateral and apical plasma membrane domains. This property has been extensively used in cell biology to investigate epithelial cell function. To date, a major limitation of this approach has been the difficulty of obta...

journal_title：European journal of cell biology

authors： Murphy C,Zacchi P,Parton RG,Zerial M,Lim F

更新日期：1997-03-01 00:00:00

abstract：:Signal transduction elements, including protein kinase C, have been identified in mammalian spermatozoa. In order to evaluate the pattern of expression and the subcellular localization of nine different protein kinase C isoforms in the course of spermatogenesis, we utilized quantitative electron microscopy immunocytoc...

journal_title：European journal of cell biology

authors： Zini N,Matteucci A,Sabatelli P,Valmori A,Caramelli E,Maraldi NM

更新日期：1997-02-01 00:00:00

abstract：:Autotetraploid (4n = 32) cells were induced in Allium cepa L. root meristems by successively treating with a multipolarizing agent in anaphase (carbetamide) and an inhibitor of cell plate formation in telophase (caffeine). This treatment produced cells with their 32 chromosomes distributed in more than two nuclei. Dur...

journal_title：European journal of cell biology

authors： Panzera F,Giménez-Abián MI,López-Sáez JF,Giménez-Martín G,Cuadrado A,Cánovas JL,De la Torre C

更新日期：1997-01-01 00:00:00

abstract：:Caldesmon and calponin are two proteins that are characteristic of vertebrate smooth muscle. In invertebrates, caldesmon has only been studied in some molluscan muscles, and no previous references to calponin have been found. The aim of this paper was to investigate the presence and distribution of caldesmon and calpo...

journal_title：European journal of cell biology

authors： Royuela M,Fraile B,Picazo ML,Paniagua R

更新日期：1997-01-01 00:00:00

abstract：:Mouse epidermal melanoblasts preferentially proliferated from disaggregated epidermal cell suspensions derived from newborn mouse skin in serum-free medium (MPM) supplemented with dibutyryl adenosine 3', 5' cyclic monophosphate (DBcAMP) and basic fibroblast growth factor (bFGF). After 12 days, almost all keratinocytes...

journal_title：European journal of cell biology

authors： Hirobe T

更新日期：1996-12-01 00:00:00

abstract：:HeLa cells growing in vitro were treated with the peptidyl aldehyde inhibitor of the chymotrypsin-like activity of the proteasome N-benzyloxycarbonyl-Ile-Glu(O-t-butyl)-Ala-leucinal (PSI). Immunofluorescence studies of treated cells revealed the formation of massive perinuclear aggregates rich in ubiquitin and proteas...

journal_title：European journal of cell biology

authors： Wójcik C,Schroeter D,Wilk S,Lamprecht J,Paweletz N

更新日期：1996-11-01 00:00:00

abstract：:In the present study, immunoelectron microscopy was used to characterize two types of coated vesicles at the trans Golgi reticulum (TGR) of rat early spermatids. Most of the coated vesicles and buds had a 18.4 +/- 0.5 nm thick electron-dense coat. The size of the vesicles without the coat was 102.6 +/- 3.6 nm. This co...

journal_title：European journal of cell biology

authors： Martínez-Menárguez JA,Geuze HJ,Ballesta J

更新日期：1996-10-01 00:00:00

abstract：:In search for the cellular mechanisms of graviperception in basidiomycete fruit bodies, nuclear and vacuole motility was investigated in the fungus, Flammulina velutipes. In this organism, hyphal nuclei are closely linked with the actin cytoskeleton, which is involved in gravity perception. Active motion of the nuclei...

journal_title：European journal of cell biology

authors： Monzer J

更新日期：1996-10-01 00:00:00

abstract：:The release of newly synthesized neuropeptides was studied in an in vitro system using the adipokinetic hormone (AKH)-producing cells of an insect (Locusta migratoria) as a model system. Tritiated phenylalanine incorporated into three hormonal neuropeptides, AKH I, II and III, was used to distinguish newly synthesized...

journal_title：European journal of cell biology

authors： Sharp-Baker HE,Oudejans RC,Kooiman FP,Diederen JH,Peute J,Van der Horst DJ

更新日期：1996-09-01 00:00:00

abstract：:The 90-kDa heat-shock protein, hsp90, is an abundant and conserved protein located predominantly in the cytoplasm. Previous reports have localized a portion of the hsp90 to microtubules and to microfilaments. Here, we show that colocalization of hsp90 with microtubules is seen after long-term but not short-term fixati...

journal_title：European journal of cell biology

authors： Czar MJ,Welsh MJ,Pratt WB

更新日期：1996-08-01 00:00:00

abstract：:Indirect evidence suggests that a chicken homologue to caudal, CHox-cad, might be involved in the development of endoderm-derived tissues and in the regeneration of the adult intestinal epithelium. Using the adult liver as an experimental system, we have investigated whether CHox-cad expression can be induced by a reg...

journal_title：European journal of cell biology

authors： Doll U,Niessing J

更新日期：1996-07-01 00:00:00

abstract：:HeLa cells were treated with different concentrations of an inhibitor of the proteasome chymotrypsin-like activity, the peptidyl aldehyde N-benzyloxycarbonyl-Ile-Glu(O-t-butyl)-Ala-leucinal (PSI). A detailed analysis, which included flow cytometry, cell counting and morphological assessment, was performed. PSI treatme...

journal_title：European journal of cell biology

authors： Wójcik C,Schroeter D,Stoehr M,Wilk S,Paweletz N

更新日期：1996-06-01 00:00:00

abstract：:The development of an efficient immune response depends on the capacity of antigen-specific lymphocytes to migrate into secondary lymphoid organs. The first step in the process of lymphocyte extravasation involves lymphocyte binding to the vascular endothelium. Although several adhesion receptors have been implicated ...

journal_title：European journal of cell biology

authors： Castro A,Bono MR,Simon V,Rosemblatt M

更新日期：1996-05-01 00:00:00

abstract：:The induction of pollen embryogenesis in Capsicum annuum L. has been studied at the cellular level using various in situ approaches with several molecular probes for DNA, RNA and proteins. The late vacuolated microspore and the young bicellular pollen grain are stages of gametophytic development in which embryogenesis...

journal_title：European journal of cell biology

authors： González-Melendi P,Testillano PS,Ahmadian P,Fadón B,Risueño MC

更新日期：1996-04-01 00:00:00

abstract：:In the sea urchin embryo there are at least two cell adhesion molecules related to mammalian cadherins, one of them, similar to E-cadherin, is expressed in embryos at very early developmental stages, the second appears at the blastula stage (G. Ghersi et al. Mech. Dev. 41, 47-55 (1993)). We show here that when sea urc...

journal_title：European journal of cell biology

authors： Ghersi G,Salamone M,Levi G,Vittorelli ML

更新日期：1996-03-01 00:00:00

abstract：:To examine the transport of the single subunits of the glycoprotein complex gp80 (clusterin, apolipoprotein J), a marker protein for apical exocytosis and main secretory product of Madin-Darby canine kidney (MDCK) cells, several mutant cDNAs were constructed and expressed in the fibroblastic baby hamster kidney (BHK-2...

journal_title：European journal of cell biology

authors： Losch A,Henkel K,Wagner M,Urban J,Koch-Brandt C

更新日期：1996-02-01 00:00:00

abstract：:In this study, we investigated the effect of plant microtubule-associated proteins (MAPs) on microtubule nucleation and growth in vitro. Since it has recently been demonstrated that plant nuclear surface acts as a microtubule-organizing center (MTOC), we tested the effects of plant MAPs using a nucleus-mediated microt...

journal_title：European journal of cell biology

authors： Stoppin V,Lambert AM,Vantard M

更新日期：1996-01-01 00:00:00

abstract：:Cartilage matrix protein (CMP), a major noncollagenous component of certain types of hyaline cartilage, is synthesized by chondrocytes in a developmentally regulated manner. In this study, we monitored the accumulation of CMP in the developing chicken limb and sternum by immunostaining. In older embryos, the specific ...

journal_title：European journal of cell biology

authors： Muratoglu S,Bachrati C,Malpeli M,Szabó P,Neri M,Dozin B,Deák F,Cancedda R,Kiss I

更新日期：1995-12-01 00:00:00

abstract：:When virgin temperature-sensitive mutant cdc28 cells of the yeast Saccharomyces cerevisiae were incubated at restrictive temperatures, they continued to grow, reaching a maximum of 3.3-fold the original size after 24 h. The protein and RNA levels increased during the first 24 h, then gradually decreased. The cells tha...

journal_title：European journal of cell biology

authors： Motizuki M,Yokota S,Tsurugi K

更新日期：1995-11-01 00:00:00

abstract：:The nuclear lamins are karyophilic proteins located at the nucleoplasmic surface of the inner nuclear membrane. We have constructed mutants immediately N-terminal to the nuclear localization signal of human lamin A to identify sites regulating the nuclear transport of the protein. Using an in vitro transport assay, we...

journal_title：European journal of cell biology

authors： Leukel M,Jost E

更新日期：1995-10-01 00:00:00

abstract：:The v-mos oncogene product has the ability to induce differentiation in human monocytic leukemia U937 cells, thereby arresting cell proliferation, and also exhibits transforming activity in mouse NIH3T3 cells. Mutation in the v-mos gene consisting of one or two amino acid substitutions in the putative ATP-binding doma...

journal_title：European journal of cell biology

authors： Kurata N,Akiyama H,Hosoya H,Aoki H,Ishikawa K,Marunouchi T

更新日期：1995-09-01 00:00:00

abstract：:Secretory granules of GH4C1 cells, a rat pituitary tumor cell line, are known to be induced by the treatment of estradiol (E2), insulin, and epidermal growth factor (EGF). We examined changes in the localization of cathepsins B, H, and L, lysosomal cysteine proteinases, in GH4C1 cells before and after hormonal treatme...

journal_title：European journal of cell biology

authors： Waguri S,Sato N,Watanabe T,Ishidoh K,Kominami E,Sato K,Uchiyama Y

更新日期：1995-08-01 00:00:00

abstract：:Filensin is a lens-specific intermediate filament protein, expressed in the lens fiber cells but not the lens epithelium. Using antibodies to filensin and the other lens intermediate filament proteins, vimentin and CP49, the codistribution of filensin with CP49 and independence of this network from the vimentin networ...

journal_title：European journal of cell biology

authors： Sandilands A,Prescott AR,Hutcheson AM,Quinlan RA,Casselman JT,FitzGerald PG

更新日期：1995-07-01 00:00:00

abstract：:Taking advantage of monoclonal antibodies raised against chick hypertrophic chondrocytes (BD5, LA5, AD2) and osteoblasts (SB1, SB2, SB3, SB5), we have investigated expression of differentiation stage-specific antigens by further differentiating hypertrophic chondrocytes. Expression of all antigens was developmentally ...

journal_title：European journal of cell biology

authors： Galotto M,Campanile G,Banfi A,Trugli M,Cancedda R

更新日期：1995-06-01 00:00:00

abstract：:Within eukaryotic cells tubulin generally exists in protein families composed of closely related isoforms generated either by differential gene transcription or by posttranscriptional and posttranslational mechanisms. In this study, immunological approaches were used to examine the contribution of posttranslational mo...

journal_title：European journal of cell biology

authors： Machell NH,Fritz L,MacRae TH

更新日期：1995-05-01 00:00:00

abstract：:In order to investigate the role of microfilaments in the crawling movements of lymphoid cells, we have analyzed the effects of botulinum C2 toxin and of cytochalasin D (cytoD) on the actin cytoskeleton and on the motility of a BW5147 T-lymphoma-derived cell line. Actin was ADP-ribosylated by C2 toxin in the living ce...

journal_title：European journal of cell biology

authors： Verschueren H,van der Taelen I,Dewit J,De Braekeleer J,De Baetselier P,Aktories K,Just I

更新日期：1995-04-01 00:00:00

abstract：:Exocytosis of synaptic vesicles (SV) results in the surface exposure of lumenal epitopes of SV proteins. We have recently described the use of antibodies directed against the lumenal N-terminus of synaptotagmin I (Sytlum-Abs) to morphologically monitor exo-endocytic recycling of SVs. We report here that a radioimmunoa...

journal_title：European journal of cell biology

authors： Mundigl O,Verderio C,Krazewski K,De Camilli P,Matteoli M

更新日期：1995-03-01 00:00:00

abstract：:We have investigated the effect of stepwise truncating the carboxyterminal domain ("tail") of the intermediate filament (IF) protein vimentin of the clawed toad, Xenopus laevis, on filament assembly in vitro and, using cell transfection, in vivo and also on the cellular topology of the structures formed. All truncatio...

journal_title：European journal of cell biology

authors： Rogers KR,Eckelt A,Nimmrich V,Janssen KP,Schliwa M,Herrmann H,Franke WW

更新日期：1995-02-01 00:00:00